Very Good EEA1 Antibody from CST

May 11, 2020

BlueskyReddit
Molecular Biology
Princeton
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

EEA1 (C45B10) Rabbit mAb

Catalog Number:

3288

Clone Number:

C45B10

Very good endosomal marker, excellent detection of large endosomes. Some background apparent.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

MDA-MB-231 BrCa

Primary Incubation

1:500

Blocking Agent

5% goat serum in PBS-T

Secondary Incubation

1:200 AL647

Tertiary Incubation

None

Detection

Confocal

Results Summary

Results showed very good signal with some slight noise and background. Excellent images for publication and no titration necessary.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Excellent endosomal marker stain

The Bad

Very slight background observed

The Bottom Line

Used to detect endosomes with excellent signal.

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