EMD Milipore
Anti-Casein Kinase 2α Antibody, 1AD9
05-1431
1AD9
Browse Similar Products: Casein Kinase 2 Alpha Immunofluorescence Antibodies
Antibody was titrated from 1:1000 to 1:100 and shown to work at higher dilutions in human breast cancer cells.
Immunofluorescence
MDA-MB-231 cells
1:100 o/n at 4C
5% goat serum
1:500 anti-mouse
None
Fluorescent confocal
Result showed the antibody detected largely nuclear CK2 at dilutions 1:100-1:250. Above 1:250 yielded a very weak signal. Overall, antibody showed good signal to noise at 1:100 and minimal background. Staining was mostly nuclear punctate.
N/A
Works well to distinguish CK2
Requires a large amount of antibody for successful stains
This was an efficient antibody that yielded publishable results by immunofluorescence. Other applications were not tried.