Affordable Grp94 Antibody, but Not Specific Enough

Rheumatology Research
Keele University
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Grp94

Catalog Number:

sc-393402

Clone Number:

H-10

Ab does not work well with cell lysates from mesenchymal stem cells and their extracellular vesicles. Lots of unspecific binding.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Umbilical Cord Mesenchymal Stem cells and extracellular vesicles

Primary Incubation

1:200, 4oC, overnight

Blocking Agent

5% skimmed milk with TTBS

Secondary Incubation

Goat anti-mouse SA HRP-conj 1:1000

Tertiary Incubation

N/A

Detection

Supersignal West Pico

Results Summary

Antibody may need to be used at a much stronger dilution. It was not detected strongly in MSC and EV samples and it showed unspecific binding across the membrane even after a 2hour block with milk and TTBS.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Low price

The Bad

Unspecific binding

The Bottom Line

Worth trying a sample first before buying as it did not work well in western blot. Bands identified at multiple weights across the membrane.

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