Bands at Different Levels on Western Blot with Calnexin Antibody

May 22, 2020

Rheumatology Research
Keele University
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Calnexin

Catalog Number:

sc-23954

Clone Number:

AF18

Unspecific binding present on membrane of western blot. Antibody showed unspecific binding

Experimental Design and Results Summary

Applications

Western Blot

Sample

Umbilical Cord Mesenchymal Stem cells and extracellular vesicles

Primary Incubation

1:200, 4oC, overnight

Blocking Agent

5% skimmed milk with TTBS

Secondary Incubation

Goat anti-mouse SA HRP-conj 1:1000

Tertiary Incubation

N/A

Detection

Supersignal West Pico

Results Summary

Antibody bound at different levels on membrane at did not show high specificity at predicted molecular weight

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Affordable

The Bad

Unspecific binding

The Bottom Line

Requires a lot of optimisation. Results not very reproducible with the ab.

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