Wonderful Anti-Human CD8 Antibody

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

BUV737 Mouse Anti-Human CD8

Catalog Number:

564628

Clone Number:

SK1

Browse Similar Products: CD8 Flow Cytometry Antibodies

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining CD8 and CD4 T cells in PBMCs that were vitro cultured. The staining shown in the unstimulated control.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC cultured for 4h

Primary Incubation

Surface staining with CD8 BUV737 2,5 in 50uL. 15' at 4

Blocking Agent

Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

CD8 can be stained very nicely, and it is conserved even after treatment with Perm II buffer for intracellular staining.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Super bright even after permeabilization

The Bad

N/A

The Bottom Line

Very good option to stain human CD8

Share your experience with other scientists. Write a Review! »

Join the discussion