Good Anti-Human CD27 Antibody

April 23, 2020

BlueskyReddit
B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Alexa Fluor® 700 Mouse Anti-Human CD27

Catalog Number:

560611

Clone Number:

M-T271

Browse Similar Products: CD27 Flow Cytometry Antibodies

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining PCs in human bone marrow.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human bone marrow

Primary Incubation

Anti-CD27 AF700, 4 C, 15 min

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

We stained CD27+CD38+ plasma cells in human bone marrow

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

It is bright

The Bad

Could be cheaper

The Bottom Line

Ok to use this anti-human CD27

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