Good for CD45RA Staining in Flow Cytometry

April 22, 2020

BlueskyReddit
Biomedical Sciences
Humanitas Clinical and Research Center
Ph.D. Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BV421 Mouse Anti-Human CD45RA

Catalog Number:

562885

Clone Number:

HI100

Browse Similar Products: CD45RA Flow Cytometry Antibodies

The antibody was used to stain CD45RA-positive cells in human bone marrow in a flow cytometry experiment.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human bone marrow

Primary Incubation

0.6 µL, 15 minutea at RT

Blocking Agent

FBS 2%

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD FACS Melody

Results Summary

This antibody can be efficently used to discriminate CD45RA-positive cells in flow cytometry.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Small volumes ofthe antibody can be used

The Bad

Brilliant Stain Buffer need to be used when the antibody is used with other BV dyes

The Bottom Line

This antibody is suggested to stain CD45RA-positive cells in flow cytometry.

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