Good Anti-Rat CD3:Alexa Fluor 700 from Bio-Rad

April 23, 2020

Medicine
Queen's University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

Mouse anti Rat CD3:Alexa Fluor 700

Catalog Number:

MCA772A700

Clone Number:

1F4

We use this antibody to detect T cells from rat blood and spleen samples for flow cytometry experiments. The antibody yields good staining that holds up well after fixation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Rat blood or splenocytes

Primary Incubation

1:10, 30 min at 4C

Blocking Agent

Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

This antibody yields good clear staining of rat T cells. However, the concentration needed for good staining is quite high. Also shipping to Canada took quite a long time (~3-4 weeks).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good staining.

The Bad

Relatively high concentration needed for optimal staining.

The Bottom Line

A good antibody, but order it well ahead of your experiment since the shipping takes a long time.

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