BV786 Mouse Anti-Rat CD8a from BD Biosciences

April 22, 2020

BlueskyReddit
Medicine
Queen's University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BV786 Mouse Anti-Rat CD8a

Catalog Number:

740913

Clone Number:

OX-8

Browse Similar Products: CD8A Flow Cytometry Antibodies

We use this antibody in flow cytometry experiments to detect CD8 T cells from rat blood and spleen samples. The antibody works reasonably well and clearly stains CD8+ T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Rat blood or splenocytes

Primary Incubation

1:100, 30 min at 4C

Blocking Agent

Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

The staining produced by this antibody is fine for most flow cytometry applications, including fresh and fixed samples. CD8+ T cells are stained clearly, although the concentration needed is fairly high.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good staining.

The Bad

Relatively high concentration needed for optimal staining.

The Bottom Line

A good antibody for a variety of flow cytometry applications using rat samples.

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