Alexa Fluor 647 Mouse Anti-Rat CD161a from BD Biosciences

Medicine
Queen's University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Alexa Fluor 647 Mouse Anti-Rat CD161a

Catalog Number:

565413

Clone Number:

10/78

Browse Similar Products: CD161A Flow Cytometry Antibodies

We use this antibody to detect NK cells in rat blood and spleen samples in flow cytometry experiments. The antibody works well and can distinguish between CD161-bright NK cells and CD161-dim monocytes.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Rat blood or splenocytes

Primary Incubation

1:400, 30 min at 4C

Blocking Agent

Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

This antibody works well to detect rat NK cells. In rat blood there are CD161-dim monocytes which can be easily distinguished from CD161-bright NK cells. The antibody works well in both fresh and fixed samples.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good staining.

The Bad

N/A

The Bottom Line

A good flow cytometry antibody to detect rat NK cells.

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