A Great Anti-Foxp3 AF 488 Antibody from BioLegend

April 10, 2020

BlueskyReddit
microbiology
NYU
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 488 anti-mouse/rat/human FOXP3 Antibody

Catalog Number:

320011

Clone Number:

25 tests

Mouse lymph node cells were stained for anti-CD4 and fix/permeabilized for intranuclear detection of Foxp3 in AF 488.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph node cells

Primary Incubation

3uL/million cells/mouse; 20 minutes; room temperature

Blocking Agent

FC block

Secondary Incubation

-

Tertiary Incubation

-

Detection

Flow Cytometer: ZE5 YETI

Results Summary

Consistent and discernible detection of intranuclear Foxp3 in lymph nodes, spleens, and organs

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Consistent, bright, easy to use

The Bad

Need 3uL/test

The Bottom Line

Great for reliable detection of Foxp3.

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