OK for CD10+ Cells Discrimination

April 10, 2020

BlueskyReddit
Biomedical Sciences
Humanitas Clinical and Research Center
Ph.D. Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Bioscences

Product Name:

APC-H7 Mouse Anti-Human CD10

Catalog Number:

655404

Clone Number:

HI10a

Browse Similar Products: C10 Flow Cytometry Antibodies

The antibody was used to stain CD10 positive cells from human bone marrow in a multicolor flow citometry panel.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human bone marrow cells

Primary Incubation

2.5 μL, 15 minutes at RT in the dark

Blocking Agent

FBS 2%

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD FACSMelody

Results Summary

The antibody performed well in staining CD10-positive cells from bone marrow.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Half of the recommended volume was used

The Bad

Very expensive and no smaller sizes available

The Bottom Line

The antibody can be used in flow cytometry/FACS applications.

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