Good Anti-CD34 Antibody for Flow Cytometry

Biomedical Sciences
Humanitas Clinical and Research Center
Ph.D. Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Bioscences

Product Name:

PE Mouse Anti-Human CD34

Catalog Number:

550761

Clone Number:

563

Browse Similar Products: CD34 Flow Cytometry Antibodies

The antibody was used in combination with other antibodies in a multicolor FACS panel. The antibody was used to sort CD34-positive cells from bone marrow.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human bone marrow cells

Primary Incubation

5 μL, 15 minutes at RT in the dark

Blocking Agent

FBS 2%

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD FACSMelody

Results Summary

The PE Mouse Anti-Human CD34 antibody efficiently stained CD34-positive cells for FACS analysis. Of note, after titration, the antibody was used at 1/4 of the recommended volume.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Efficient discrimination of CD34+ cells

The Bad

Only tested for flow citometry

The Bottom Line

The antibody allowed the discrimination and sorting of CD34+ cells from bone marrow.

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