BD Bioscences
Anti-Human FITC Lineage Cocktail 1 (lin 1) (CD3, CD14, CD16, CD19, CD20, CD56)
340546
MφP9
This antibody was used to stain lymphocytes, monocytes, eosinophils, and neutrophils and sort lineage-negative bone marrow cells by FACS.
Flow Cytometry
Human bone marrow cells
10 μL, 15 minutes at RT in the dark
FBS 2%
None
BD FACSMelody
The antibody cocktail allows the staining of lymphocytes, monocytes, eosinophils, and neutrophils. It was used to efficiently exclude these cells from the sorted population.
N/A
Works best at half of the suggested volume
Still quite expensive
Suggested to exclude lineage positive cells in cell sorting.