Thermo Fisher Scientific
CD34 Monoclonal Antibody (4H11), PE-Cyanine7
25-0349-41
4H11
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CD34 is expressed on human hematopoietic stem cells, which are crucial for the generation of bone marrow elements. In our research we identify these cells to look at their expression of other markers. We need a good antibody conjugated to a fluorochrome that is not one of the common ones (FITC, PE, APC), so we got this PE-Cy7 antibody.
Flow Cytometry
Human bone marrow specimen
5uL antibody at 4°C for 15 min, followed by wash and lyse
None
Flow Cytometry (PE-Cy7 channel = 780/60)
The results were acceptable. At 5uL the separation of stem cells from non-stem cells was OK. Even at 2.5uL, the resolution was seen, but the two peaks were too close to each other, which is why we selected 5uL. It did lead to some spread into the PE channel, which was expected, but this made the resolution in that channel harder.
N/A
Bright signal.
Lots of spillover/spread into PE channel
The antibody worked, and we will be using it. There might be better options out there!