White Blood Cell Depletion from RBC Pellets Using Plasmodipur Filters

April 07, 2020

BlueskyReddit
New York University
Biology
Research Scientist

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Company:

Cedarlane

Product Name:

Plasmodipur Filter

Catalog Number:

8011FILTER25U

Various approaches can be used to deplete white blood cells from red blood cell pellets. One such approach is a filtration unit called Plasmodipur. The filter attaches to a syringe and through mechanical force allows passage of the RBCs through the filter while retaining the undesired white blood cells.

Experimental Design and Results Summary

Application

Removal of white blood cells from RBC pellets

Starting Material

Whole blood or WBC contaminated RBC pellets.

Tips

The filters are best used alongside twist lock syringes.

Results Summary

The successful removal of contaminating white blood cells from RBC pellets using Plasmodipur filters has allowed us to focus downstream molecular biology and sequencing projects. We have confirmed the utility of this product against other depletion methods such as column filtration.

DOI or PMID #

N/A

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Summary

The Good

Easy to use; Reliable results; Amenable to any setting (field, lab).

The Bad

The filters are expensive; No discount if multiple boxes are purchased from the company.

The Bottom Line

We utilize the Plasmodipur filter to remove white blood cells from parasite infected RBC pellets. This allows us to reduce the amount of human genomic material in our samples which has huge implications for our downstream molecular biology and sequencing projects.

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