Good FLAG Antibody from MilliporeSigma

April 08, 2020

Orthopedic surgery
Johns Hopkins University School of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

MilliporeSigma

Product Name:

ANTI-FLAG antibody produced in rabbit

Catalog Number:

F7425

Clone Number:

Polyclonal

Browse Similar Products: FLAG Western Blot Antibodies

I used this antibody to check the expression of FLAG in cells that were transfected by plasmids or infected by virus.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse CAD cell line

Primary Incubation

1:500, overnight at 4°C

Blocking Agent

TBST (0.1%) containing 5% blotting-grade blocker

Secondary Incubation

1:10000, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

KwikQuantTM Imager

Results Summary

This antibody is sensitive and specific and the band locates at the right place.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Sensitive and specific

The Bad

N/A

The Bottom Line

I would recommend using this antibody to check the expression of FLAG in cells by western blot experiments.

Share your experience with other scientists. Write a Review! »

Join the discussion