Works to Stain Ki-67, but It's Not the Best

Genetics
Emory University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Purified Mouse Anti-Ki-67

Catalog Number:

550609

Clone Number:

B56

Browse Similar Products: Ki-67 Immunofluorescence Antibodies

The KI-67 antibody did stain proliferating cells, but required a high amount of antibody and still did not stain the best.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Cortical Organoid

Primary Incubation

1:50 dilution 4 degrees overnight

Blocking Agent

5% Donkey Serum + 0.1% Triton X-100 in PBS

Secondary Incubation

Donkey anti Mouse (488) 1:1500 2 hours at room temp

Tertiary Incubation

N/A

Detection

Fluorescent Microscope

Results Summary

KI-67 did stain my cells, but the results were not very high quality.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Stains proliferating cells

The Bad

Requires a large amount of antibody

The Bottom Line

This antibody will work, but need a lot of it and doesn't stain the best.

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