Agilent Dako
Anti-Glial Fibrillary Acidic Protein (GFAP)
Z033429-2
Polyclonal
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We used the anti-GFAP antibody to stain for astrocytes and the stains come out beautiful with the recommended dilution, so there was little optimization that needed to be done.
Immunofluorescence
Immunopanned cortical organoids
1:1500 overnight at 4C
5% Donkey Serum + 0.3% Triton X-100 in PBS
Anti Rabbit (488) for 2 hours at room temp in blocking
N/A
Fluorescent Microscope
We immunopanned day 82 cortical organoids using anti-HepaCAM. We then plated the cells on coverslips and stained with anti-GFAPand DAPI. You can see the long projections of the astrocytes by staining with GFAP (in green).
Low optimization required
Antibody is expensive
The antibody works extremely well and you can get beautiful stains from it.