This Phospho-AKT1 Antibody from Thermo Works Great

March 18, 2020

BlueskyReddit
Internal Medicine
East Tennessee State University
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Thermo Fisher Scientific

Product Name:

Phospho-AKT1 (Ser473) Monoclonal Antibody (SDRNR), APC, eBioscience™

Catalog Number:

17-9715-42

Clone Number:

SDRNR

We used this antibody to check the difference in phospho-AKT1 after HIV infection in human CD4 T cells. The antibody works well and gives good separation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human CD4 T cells

Primary Incubation

1:50, 1X perm buffer

Blocking Agent

1X perm buffer

Secondary Incubation

N/A

Tertiary Incubation

1:50, 45min

Detection

BD C6 Plus

Results Summary

pAKT expresses less after HIV infection comparing with uninfected.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Separates well!

The Bad

Not found.

The Bottom Line

Easy to operate, separated well.

Share your experience with other scientists. Write a Review! »

Join the discussion