Works Really Nicely, Used at 1:300

March 20, 2020

Cell Bio
Inhibrx
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-human CD16 Antibody

Catalog Number:

302038

Clone Number:

3G8

I was trying to stain for eosinophils in RBC-lysed whole blood. Eosinophils are CD16-CCR3+.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Cells in RBC-lysed whole blood

Primary Incubation

30' 4*C

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometer

Results Summary

This antibody made it easy to distinguish between CD16+ and CD16- populations; there is a very clear distinction between the two. Additionally it can be used at a fairly dilute concentration.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works great

The Bad

Nothing bad

The Bottom Line

This is a really nice antibody.

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