Competent E. Coli Cells for Protein Expression

Harvard Medical School
Cell Biology
Postdoctoral Fellow

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Quality of Results

Ease-of-Optimization

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Company:

MilliporeSigma

Product Name:

BL21(DE3)pLysS Singles Competent Cells

Catalog Number:

70236-3

We use this strain to express recombinant proteins for purification. It contains tRNAs for rare codons, so the expression of human protein is more efficient.

Experimental Design and Results Summary

Application

Protein expression and purification

Starting Material

competent cell and plasmid

Tips

The cells are very efficient, only need to use 5 uL to do transformation

Results Summary

I had a lot of success to express and purify high yeild of protein using this cell strain.

DOI or PMID #

N/A

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Summary

The Good

High yeild of protein expressed

The Bad

Expensive

The Bottom Line

I would recommend using this competent cell to express and purify recombinant proteins.

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