Illumina
Miseq reagent ki v3
MS-102-3001
We use this kit to do the sequencing for our CRISPR screen samples. It provides enough depth for the anlaysis. It also takes much shorter time than sending the samples out.
Next generation sequencing
PCR sample from screen
Need to accurately measure the DNA sample concentration
We got deep sequencing results with sufficient reads and high sucessful rate.
N/A
Reliable, high quality, efficient, fast
Expensive
I would recommend this sequencing kit for sequencing of small libraries, such as for small scale CRISPR screen.