GATA4 Antibody for Flow Cytometry Analysis

February 25, 2020

Cell Biology
Harvard Medical School
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Alexa Fluor® 647 Mouse Anti-GATA4

Catalog Number:

560400

Clone Number:

L97-56

This antibody is a good antibody for GATA4 with APC fluorofore that can be applied to flow cytometry analysis

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Endoderm cells

Primary Incubation

1:50, room temperature, 45 minutes

Blocking Agent

FACS buffer

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence

Results Summary

Using this antibody, we get strong and specific signal for GATA4 which is a marker for endoderm cells differentiated from embryonic stem cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific signal, easy to optimize

The Bad

None

The Bottom Line

This is a reliable choice of flow cytometry antibody for GATA4

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