Works OK for Detecting NEDD8 in Western Blot

February 26, 2020

Cell Biology
Harvard Medical School
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

NEDD8 (19E3) Rabbit mAb #2754

Catalog Number:

2754

Clone Number:

19000

This NEDD8 antibody works fine to detect endogenous NEDD8 and NEDDylated proteins in the cell. It is not super specific.

Experimental Design and Results Summary

Applications

Western Blot

Sample

H9 cell line

Primary Incubation

1:1000 4 ℃ overnight

Blocking Agent

5% milk

Secondary Incubation

1:10000, room temperature, 1 hour

Tertiary Incubation

None

Detection

Fluorescence

Results Summary

Observed specific bands corresponding to NEDD8 protein and the neddylated proteins. There are also non-specific signals.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Can detect NEDD8 with high sensitivity

The Bad

Not specific

The Bottom Line

I would recommend this antibody since it is relatively good to detect NEDD8 for cell based assay or in vitro assays

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