Non-Specific CX3CL1 Antibody from Invitrogen

March 18, 2020

BlueskyReddit
Orthopedic surgery
Johns Hopkins University School of Medicine
Postdoctoral Researcher

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Quality of Results

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Company:

Invitrogen

Product Name:

CX3CL1 (Fractalkine) Polyclonal Antibody

Catalog Number:

14-7986-81

Clone Number:

Polyclonal

I used this antibody to detect the expression of CX3CL1 in mouse dorsal root ganglion by western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse dorsal root ganglion

Primary Incubation

1:500, overnight at 4°C

Blocking Agent

TBST (0.1%) containing 5% blotting-grade blocker

Secondary Incubation

1:10000, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

KwikQuantTM Imager

Results Summary

There’s another band in addition to the target band around 52KD ladder. This antibody is not specific.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Sensitive

The Bad

Non-Specific

The Bottom Line

I would not recommend using this antibody to detect the expression of CX3CL1 by western blot.

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