Multiple Bands with This CD68 Antibody from Abcam

March 17, 2020

BlueskyReddit
Orthopedic surgery
Johns Hopkins University School of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Anti-CD68 antibody

Catalog Number:

ab125212

Clone Number:

Polyclonal

I used this antibody to detect the expression of CD68 in mouse dorsal root ganglion by western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse dorsal root ganglion

Primary Incubation

1:1000, overnight at 4°C

Blocking Agent

TBST (0.1%) containing 5% blotting-grade blocker

Secondary Incubation

1:10000, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

KwikQuantTM Imager

Results Summary

There are multiple bands, the most clear one is about 72 KD.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Sensitivity

The Bad

Non-specific

The Bottom Line

I would not recommend using this antibody to detect the expression of CD68 by western blot.

Share your experience with other scientists. Write a Review! »

Join the discussion