Specific Antibody against ATF-3 from Santa Cruz

March 16, 2020

BlueskyReddit
Orthopedic surgery
Johns Hopkins University School of Medicine
Postdoctoral Researcher

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Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

ATF-3 Antibody (44C3a)

Catalog Number:

sc-81189

Clone Number:

44C3a

I used this antibody to detect the expression of Atf3 in mouse dorsal root ganglion by western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse dorsal root ganglion

Primary Incubation

1:500, overnight at 4°C

Blocking Agent

TBST (0.1%) containing 5% blotting-grade blocker

Secondary Incubation

1:10000, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

KwikQuantTM Imager

Results Summary

This antibody is specific. This primary antibody is used in 1:500, I still need to use the super signal ECL to see the band. One possible reason is the low amount of expressed Atf3.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This antibody is specific.

The Bad

N/A

The Bottom Line

I would recommend using this antibody to detect the expression of Atf3.

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