Excellent Technical Reproducibility - Beta-Amyloid ELISA Kit

Massachusetts General Hospital
Neurology
Principal Investigator

Overall

Quality of Results

Ease-of-Optimization

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Company:

EUROIMMUN

Product Name:

Human Beta-Amyloid (1-40) ELISA Kit

Catalog Number:

EQ 6511-9601-L

We tested EuroImmun kits as part of testing systems and assays for ATN determination in CSF. We found them to be robust and reproducible.

Experimental Design and Results Summary

Application

Establishing ATN values

Starting Material

Human CSF

Tips

We have used both the Analyzer I and hand preparation for this kit and get excellent results with both

Results Summary

For the AB 1-40 kit we had a mean duplicate sample CV of 1.7 %, and a plate to plate standard control mean CV of 1.6 %.

DOI or PMID #

N/A

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Summary

The Good

High technical reproducibility, clear instructions, ability to automate using Analyzer I instrument

The Bad

Standard AnalyzerI results output is clunky, mixes text and numeric.

The Bottom Line

This kit is robust, reproducible and easy to use in the lab. It can be used without the purchase of an expensive instrument, making it accessible to labs with a standard colorimetric plate reader.

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