Excellent Technical Reproducibility - Beta-Amyloid ELISA Kit

Massachusetts General Hospital
Neurology
Principal Investigator

Overall

Quality of Results

Ease-of-Optimization

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Company:

EUROIMMUN

Product Name:

Human Beta-Amyloid (1-42) ELISA Kit

Catalog Number:

EQ 6521-9601-L

As part of testing multiple assay systems to define ATN values for CSF samples in our laboratory, we tested the EuroImmun Human Beta-Amyloid (1-42) ELISA Kit. We found the kit to be easy to use and the results robust and reproducible.

Experimental Design and Results Summary

Application

Establishing ATN values

Starting Material

Human CSF

Tips

We have used both the Analyzer I and hand preparation for this kit and get excellent results with both

Results Summary

When using this kit for large studies in our lab we find within sample CV of 2.2% and a plate to plate CV of 1.2% for our standard samples. No samples we have tested have had values outside the assay range. Please note that these values come from a previous iteration of the kit before the AB42 global standard was incorporated to the assay.

DOI or PMID #

N/A

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Summary

The Good

High technical reproducibility, clear instructions, ability to automate using Analyzer I instrument

The Bad

Standard AnalyzerI results output is clunky, mixes text and numeric.

The Bottom Line

Excellent robust assay.

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