New England Biolabs
NEBuilder® HiFi DNA Assembly Cloning Kit
E5520S
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I use this in replacement of Gibson assembly kit.
DNA fragment assembly (4 fragments assembly is possible!)
Several DNA fragments
I used blunt end DNA fragments (total 4) and it worked very well
I use a very small volume per reaction (~3 ul each) and it still works well.
N/A
Great efficiency! I have never failed using this reagent
A bit expensive
I highly recommend this when cloning!