The Good
Kits are available with variant numbers of unique barcode adapters (e.g. 6, 12, 24, 48, etc.); All vials contain enough reagent for up to 8 reactions; The barcodes can be used with or without additional PCR amplification cycles and are therefore amenable to a wide range of sample concentrations.
The Bad
The barcodes are expensive.
The Bottom Line
We endorse the use of the NEXTflex DNA barcodes in the NEXTflex Rapid DNA-Seq library preparation protocol. We have had success with sequencing samples on the low end of the input range (e.g. 1 ng) and are pleased that this kit gives us the ability to multiplex our Illumina sequencing runs via a simple and intuitive workflow.