NEXTflex PCR-Free Barcodes for Multiplexing PCR-Free Genomic Library Preparations

March 26, 2020

BlueskyReddit
New York University
Biology
Research Scientist

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Company:

Bioo Scientific Corporation

Product Name:

NEXTflex PCR-Free Barcodes

Catalog Number:

NOVA-514110/1/2/3

The NEXTflex PCR-Free barcodes are best used with the NEXTflex PCR-Free DNA-Seq Kit and come in quantities of 6, 12, 24, and 48 unique barcode adapters. Each unique adapter contains enough material for 8 reactions, and the instructions for use are clearly defined in the user manual of both the DNA-Seq Kit and the Barcode Kit.

Experimental Design and Results Summary

Application

Barcoding genomic DNA libraries for multiplexed sequencing

Starting Material

Genomic DNA (500 ng to 3 ug of starting material required)

Tips

This specific kit is validated for use with Illumina platforms only.

Results Summary

Successful adapter ligation can be determined by quantitative PCR (concentration) and fragment size determination (TapeStation or equivalent method).

DOI or PMID #

N/A

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Summary

The Good

High quality results; Time efficient and intuitive workflow; Volume provided in kit allows for many reactions to be performed from a single order.

The Bad

Can only be used with PCR-free DNA-seq Kit (e.g. not amenable to library preparation protocols requiring PCR amplification).

The Bottom Line

The NEXTflex PCR-free barcodes are the perfect complement to the NEXTflex PCR-free DNA-Seq Kit for multiplexed genomic sequencing of high concentration samples on Illumina platforms. The workflow is seamless.

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