Good Flow Cytometry Antibody for Mouse CD138

March 27, 2020

BlueskyReddit
Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

APC anti-mouse CD138 (Syndecan-1) Antibody

Catalog Number:

142506

Clone Number:

281-2

I used this antibody to stain CD19low CD138+ plasma B-cells in mouse. This antibody stained well and showed distinguished CD19low CD138+ population when used in combination with CD19 antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes and lymph node cells

Primary Incubation

1:100 for 90 min at 4 degree

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

This clone works well and gives reproducible results.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Cheap and reproducible results

The Bad

May require effort for optimization

The Bottom Line

It's worth buying this antibody

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