Good Flow Cytometry Antibody for Mouse GL-7

March 25, 2020

BlueskyReddit
Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Thermo Fisher Scientific

Product Name:

GL7 Monoclonal Antibody (GL-7 (GL7)), PE, eBioscience™

Catalog Number:

12-5902-80

Clone Number:

GL-7 (GL7)

I used this antibody to stain CD19+B220+GL-7+ germinal center B-cells in mouse. This antibody stained well and showed distinguished CD19+B220+GL-7+ population when used in combination with CD19 and B220 antibodies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes and lymph node cells

Primary Incubation

1:100 for 90 min at 4 degree

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

This antibody stained well and showed distinct population. I am able to get reproducible results.

DOI or PMID #

N/A

Additional Notes

GC B-cells may not be abundant in normal mice, but can be seen in infected/immunized mice.

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Summary

The Good

Good specificity and ease of optimization

The Bad

May require some effort for optimization

The Bottom Line

It's worth buying this antibody.

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