Good Antibody for Mouse CD3 Staining

March 02, 2020

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Thermo Fisher Scientific

Product Name:

CD3e Monoclonal Antibody (145-2C11), PE-Cyanine7, eBioscience™

Catalog Number:

25-0031-82

Clone Number:

145-2C11

I use this antibody to stain mouse T-cells in the spleen. It works great every time and gives a clear distinct T-cell population.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:300 for 1h at 4 degree

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

This antibody stained well and showed distinct population. I am able to get reproducible results.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Ease of optimization and reproducible results

The Bad

Expensive

The Bottom Line

It's worth buying this antibody

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