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APC anti-mouse B7-H4 (B7S1, B7X) Antibody
139408
HMH4-5G1
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With this experiment I was hoping to determine an optional dilution to use this antibody at where I would get no background but still a nice signal. But I don't want too bright of a signal for compensation reasons. 1:200 seemed to be a good dilution.
Flow Cytometry
293FS transfected (or not) with muB7H4
30 minutes 4*C
N/A
Flow cytometer
This antibody binds really nicely. I would use it at 1:200, but you could probably dilute it more than that if your target expresses a lot of B7H4 (for example, the binding is detectable on these transfected 293FS even at 1:700)It has some nonspecific binding if you use it at 1:50 or greater, so I just make sure to dilute it more than that.
Use between 1:100 - 1:300
Nice strong signal
Some nonspecific binding if used too concentrated
Good antibody that binds muB7H4 nicely even when used at fairly dilute concentrations.