Rabbit Anti-GFAP Polyclonal Antibody

March 04, 2020

BlueskyReddit
Van Andel Research Institute
Postdoctoral Researcher

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Quality of Results

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Company:

Agilent

Product Name:

Anti-Glial Fibrillary Acidic Protein, Polyclonal,(GFAP) antibody

Catalog Number:

Z0334

Clone Number:

Polyclonal

Antibody works well in rat and mouse primary astrocyte cultures. Cultures fixed on glass coverslips with 4%PFA. Used GFAP antibody at 1:2500 with at 4 degrees overnight.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Rat or mouse primary cortical cultures or primary astrocyte cultures from cortex

Primary Incubation

1:2500 at 4 degrees overnight

Blocking Agent

Bovine Serum Albumin, Goat Serum and Horse Serum

Secondary Incubation

Goat anti rabbit alexa fluorphores 1:500 room temp for 2 hours

Tertiary Incubation

None

Detection

Epifluorescent scope or laser scanning confocal

Results Summary

Antibody works great! Strong and specific signal in cultured astrocytes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Specific signal; and strong

The Bad

I haven't tested it for IHC

The Bottom Line

Works well for mouse and rat primary cultures

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