Santa Cruz Biotechnology
Alix (1A12) Antibody
sc-53540
1A12
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Browse Similar Products: Alix Western Blot Antibodies
I used this antibody to identify Alix in extracellular vesicle samples and cells from umbilical cord mesenchymal stem cells. I had to use a stronger concentration 1:100 dilution to get the appearance of bands. The bands were smudged but clearly visible.
Western Blot
Umbilical Cord Mesenchymal Stem cells and extracellular vesicles
1:100, 4oC, overnight
5% skimmed milk
Goat anti-mouse SA HRP-conj 1:1000
N/A
Supersignal West Pico
Bands visible between 90-100kDa. Some smudged. 5-7 min incubation in West Pico required
Affordable
High Concentration required
It works well but other companies provide stronger Alix antibodies with clearer bands.