Excellent CD279 Antibody When Used at 1:100

March 09, 2020

Cell Bio
Inhibrx
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-human PD1

Catalog Number:

329906

Clone Number:

EH12.2H7

This antibody clearly differentiates the distinct PD1+ population when used to stain T cells that were activated for 2 days with CD3 (OKT3).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Purified T cells activated for 2 days with plate bound OKT3

Primary Incubation

30 minutes at 4*C

Blocking Agent

No blocking

Secondary Incubation

No secondary incubation

Tertiary Incubation

No tertiary incubation

Detection

Detected with Novocyte (flow cytometer)

Results Summary

This PE conjugated PD1 antibody clearly shows a distinctive PD1+ population, which is as expected since stimulated T cells should upregulate PD1.

DOI or PMID #

N/A

Additional Notes

I used at 1:100, but you can probably dilute it more.

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Summary

The Good

Works very well

The Bad

They suggest using a lot, you definitely can dilute it more. Its a tiny bit annoying that the isotype control (mIgG2a) from biolegend for this antibody is a different stock concentration.

The Bottom Line

A good antibody that makes it very easy to detect your PD1+ population.

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