HRP Anti-His Tag Antibody from BioLegend

Lymphocyte Signalling
Babraham Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

HRP anti-His Tag Antibody

Catalog Number:

652503

Clone Number:

J099B12

I have a His-tag on human and mouse proteins and this antibody worked well at various dilutions of antibody as well as supernatants.

Experimental Design and Results Summary

Applications

ELISA

Sample

Supernatants

Primary Incubation

Coated plate with antibody of interest overnight at 4 degrees

Blocking Agent

2% BSA

Secondary Incubation

Samples were incubated at 37C for 1 hour

Tertiary Incubation

Anti-histag HRP was incubated for 1 hour at room temperature

Detection

TMB substrate was used to develop colour, stopped with 2N H2SO4/ 2N HCL. Read at A450nm

Results Summary

Dose response was obtained after incubating the anti-histag antibody at dilutions 1:500, 1:1000, 1:2500, and 1:5000. From this I have been using 1:5000 dilution for the rest of my experiments.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Worked well

The Bad

Nothing

The Bottom Line

Easy to use with various dilutions. Gave dose-dependent results.

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