Detects Human p38 Full Length

Pharmacology
Duke University
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

p38 MAPK

Catalog Number:

8690

Clone Number:

D13E1

This product is used to detect non-phosphorylated p38 in human extracts. We used it in MDA_MB-231 cancer cells stimulated with TNF, and it worked well enough.

Experimental Design and Results Summary

Applications

Western Blot

Sample

MDA_MB-231

Primary Incubation

1:500

Blocking Agent

Normal Donkey Serum

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

This antibody worked well to detect p38 in MDA_MB-231 cancer cells stimulated with TNF.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Detects with minimal off target

The Bad

Need some optimization.

The Bottom Line

This works well but one must optimize the concentration and blocking buffers first.

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