A Good CD5 Antibody from BioLegend

Department of Pathology
NYU Langone Health
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-mouse CD5 Antibody

Catalog Number:

100626

Clone Number:

53-7.3

My work is focused on the exploration of mechanisms behind inflammation in cancer initiation and progression; signaling pathways that regulate autophagy and autophagy-related proteins in the inflammatory tumor microenvironment.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mice tissue digests

Primary Incubation

Primary antibody was incubated at 1:100 dilution at 4C

Blocking Agent

Before staining, cells were blocked with (FACS buffer + 2% BSA + 1:100 Anti-Mouse CD16/CD32)

Secondary Incubation

No secondary incubation

Tertiary Incubation

No tertiary incubation

Detection

Flow cytometry

Results Summary

We observed a significant surface expression of CD5 protein in our experiments in murine cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This antibody is good for CD5 protein detection by flow cytometry

The Bad

N/A

The Bottom Line

This antibody is good for the detection of CD5 protein in murine samples with high specificity and low background. Best results at dilution of 1:100.

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