CCR7 Antibody from BioLegend

Department of Pathology
NYU Langone Health
Graduate Student

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Quality of Results

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Company:

BioLegend

Product Name:

APC anti-mouse CD197 (CCR7) Antibody

Catalog Number:

120108

Clone Number:

4B12

My work is focused on the exploration of mechanisms behind inflammation in cancer initiation and progression; signaling pathways that regulate autophagy and autophagy-related proteins in the inflammatory tumor microenvironment.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mice tissue digests

Primary Incubation

Primary antibody was incubated at 1:100 dilution at room temperature

Blocking Agent

Before staining, cells were blocked with (FACS buffer + 2% BSA + 1:100 Anti-Mouse CD16/CD32)

Secondary Incubation

No secondary incubation

Tertiary Incubation

No tertiary incubation

Detection

Flow cytometry

Results Summary

We observed a significant surface expression of CCR7 protein in our experiments in murine tissue cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This antibody is good for CCR7 protein detection by flow cytometry

The Bad

Only stains at room temperature

The Bottom Line

This antibody is good for the detection of CCR7 protein in murine samples with high specificity and low background.

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