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Anti-mouse/human B220 Antibody
103212
RA3-6B2
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My work is focused on the exploration of mechanisms behind inflammation in cancer initiation and progression; signaling pathways that regulate autophagy and autophagy-related proteins in the inflammatory tumor microenvironment.
Flow Cytometry
Mice tissue digests
Primary antibody was incubated at 1:100 dilution at 4C
Before staining, cells were blocked with (FACS buffer + 2% BSA + 1:100 Anti-Mouse CD16/CD32)
No secondary incubation
No tertiary incubation
Flow cytometry
We observed a significant surface expression of B220 protein in our experiments on cells.
N/A
This antibody is good for B220 protein detection by flow cytometry
This antibody is good for the detection of B220 protein in murine samples with high specificity and low background. Best results at dilution of 1:100.