Miltenyi Biotec
Anti-human CD45 VioBlue
130-113-684
5B1
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This antibody was used to stain for leukocytes in human peripheral blood. It was chosen because it is a commonly used clone for CD45, VioBlue works well with other fluorophores in the staining panel, and Miltenyi has a good reputation for making quality flow cytometry antibodies.
Flow Cytometry
Whole blood
5 ul per test in 100uL staining volume for 15 minutes at room temperature.
FcBlock + 0.2% BSA
N/A
BD LSR II
CD45 staining was excellent. Positive cells were fully resolved from negative.
Works well. Reproducible results.
Will continue to use this antibody.