Expression of MYBPC3 in Disease Condition

SCHOOL OF VETERINARY MEDICINE AND BIOMEDICAL SCIENCES
University of Nebraska
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Santa Cruz Biotechnology

Product Name:

MYBPC3

Catalog Number:

SC-67353

Clone Number:

G-7

Cardiac myosin binding protein C (MYBPC3) is the most commonly mutated gene associated with hypertrophic cardiomyopathy (HCM). Haploinsufficiency of full-length MYBPC3 and disruption of proteostasis have both been proposed as central to HCM disease pathogenesis. Here we checked in disease condition increased level of MYBPC3 protein levels.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Animal tissue cells, RAW 264.7 cells

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1X TBST

Blocking Agent

5% skim milk

Secondary Incubation

1:5000 dilution in 5% skim milk prepared in 1X TBST

Tertiary Incubation

N/A

Detection

ECL

Results Summary

We found increased levels of MYBPC3 protein in disease condition.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No background

The Bad

None

The Bottom Line

This antibody is very good. Highly recommended.

Share your experience with other scientists. Write a Review! »

Join the discussion