Expression of CD196 Using Flow Cytometry

SCHOOL OF VETERINARY MEDICINE AND BIOMEDICAL SCIENCES
University of Nebraska
Postdoctoral Researcher

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Company:

BioLegend

Product Name:

CD196 antibody

Catalog Number:

129803

Clone Number:

29-2L17

CD196, is a chemokine receptor that is expressed on immature dendritic cells, B lymphocytes, and memory T cells. It can function to mediate arrest of T cells on dermal endothelial cells and is highly expressed on T cells residing in both normal and psoriatic skin. Here we performed our study on the immunization of isotopes in mice, then we looked at the expression of CD196.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Animal tissue cells, lymphocyte cells

Primary Incubation

1:100

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

We found nice expression of CD196 using flow cytometry.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong fluorescent signal

The Bad

None

The Bottom Line

This antibody has an awesome signal. Highly recommended.

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