ß-Actin as a Loading Control for Immunoblotting

March 30, 2020

Molecular Biology and Biochemistry
Med Uni Graz
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

MilliporeSigma

Product Name:

Anti-β-Actin antibody, Mouse monoclonal

Catalog Number:

A1978

Clone Number:

AC-15

β-Actin was used in many immunoblotting experiment as a loading control.

Experimental Design and Results Summary

Applications

Western Blot

Sample

BV2 Microglia cells

Primary Incubation

1:5000, O/N, +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 anti mouse

Tertiary Incubation

N/A

Detection

ECL

Results Summary

No changes in the expression of β-Actin was observed between different samples.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works as expected

The Bad

N/A

The Bottom Line

A very good loading control for western blot experiments.

Share your experience with other scientists. Write a Review! »

Join the discussion