Detection of SMA Expression in Mouse Brain

Molecular Biology and Biochemistry
Med Uni Graz
Research Scientist

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Company:

Abcam

Product Name:

Anti-alpha smooth muscle Actin antibody

Catalog Number:

ab5694

Clone Number:

Polyclonal

The brains from perfused mice were collected and homogenised in order to be used for immunoblotting. The purpose of the experiment was to detect possible changes in SMA expression between brains from healthy mice and mice with a brain tumor.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse brain

Primary Incubation

1:1000, O/N, +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 anti rabbit

Tertiary Incubation

N/A

Detection

ECL

Results Summary

The samples from mice with a brain tumor were compared with samples from healthy mice. Increased expression of SMA was observed in the brains with a tumor compared to healthy controls.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works very well

The Bad

N/A

The Bottom Line

A good antibody for detecting SMA expression in brain samples.

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